старший научный сотрудник
кандидат биологических наук
Nikiforov Nikita
1
Curriculum Vitae
Personal details
Name
Nikiforov Nikita
PhD
Date and place of birth
October 31, 1989,
Birsk, Bashkortostan, Russia
Private address Kosygina str. 13, app 274,
119334 Moscow, Russia
Mobile phone: +79190577611 (Moscow)
e-mail:
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Education and career
2016 – Present time Senior researcher at the "National Medical
Research Center of Cardiology" of the Ministry
of Health of the Russian Federation
http://www.cardioweb.ru/
PhD project
2012 – 2017 PhD project performed at the "National Medical
Research Center of Cardiology" of the Ministry
of Health of the Russian Federation Thesis title:
“Study of monocyte/macrophage activation
profile in the interaction with low density
lipoproteins”
Supervisor Dr. Igor Sobenin
2012 – 2015 PhD student at the "National Medical
Research Center of Cardiology" of the Ministry
of Health of the Russian Federation
http://www.cardioweb.ru/
2014 – Present time Senior researcher at the Institute of General
Pathology and Pathophysiology of the Russian
Academy of Medical Sciences
http://www.niiopp.ru/
2006 – 2012 Student at the Moscow State University of
Physics and Technology. Department of
Molecular and Biological Physics
http://mipt.ru/
Nikiforov Nikita
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Master project
June 2011 – April 2012 Master project performed at Heidelberg
University, Medical Faculty Mannheim,
Germany, Laboratory for cell and molecular
Biology of Innate Immunity. Thesis title:
“Analysis of endocytosis and patocytosis of
disperse and aggregated modified lipoproteins
in differently activated primary macrophages.”
Supervisor Prof. Dr. Julia Kzhyshkowska
http://www.umm.uni-heidelberg.de/index_e.html
Bachelor project
Sep. 2009 – May 2010 Bachelor project performed at Institute of
Molecular Biology, Russian Academy of
Science, Moscow. Thesis title: “Influence of the
hypoxia on the differentiation and proliferation of
mouse embryonic and postnatal cells”
Supervisor Prof. Dr. Yegor Yegorov
http://www.eimb.ru/
2002 – 2006 Graduated from lyceum 1 specialized in
mathematics, Birsk, Bashkortostan, Russia
(silver medal)
2002 General School 7, Birsk, Bashkortostan, Russia.
All scores excellent
1996 – 2002 General School 9, Birsk, Bashkortostan, Russia.
Awards and prizes for scientific activity
Young Investigator Award, Winner - Oral, 11th Congress of Asian Pacific Society of
Atherosclerosis & Vascular Disease, 2018
Additional research experience
May 2010 – 2015 Research project at Institute for Atherosclerosis
Research (Skolkovo Innovative Centre)
Additional courses
Nov 2011 The Russian-Scandinavian School “Gene
diagnostics of atherosclerosis. Mitochondrial
genome”
Languages Russian – mother language
English – intermediate level
Methodological skills 1. Immunological methods: Western blot,
immunofluorescence and confocal fluorescent
microscopy, ELISA, immunohistochemistry.
2. Protein techniques: VLDL, LDL, HLDL
isolation.
3. Cell culture: subculturing and maintainance of
Nikiforov Nikita
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cell lines, isolation of monocytes from buffy
coats.
4. Functional assays: phagocytosis and
endocytosis assays.
5. Animal handling: isolation of bone marrow
stem cells from mice.
Scientific interests
Atherosclerosis, general immunology, immunoregulation, plasticity of macrophage
phenotype, M1/M2 reprogramming of macrophage phenotype, receptors of innate
immunity, role of low-density lipoproteins in atherosclerosis processes.
Publications
1. The Link between Chronic Stress and Accelerated Aging. Yegorov YE,
Poznyak AV, Nikiforov NG, Sobenin IA, Orekhov AN. Biomedicines. 2020 Jul
7;8(7):E198. doi: 10.3390/biomedicines8070198. PMID: 32645916
2. Medicinal Plants as a Potential and Successful Treatment Option in the
Context of Atherosclerosis. Kirichenko TV, Sukhorukov VN, Markin AM,
Nikiforov NG, Liu PY, Sobenin IA, Tarasov VV, Orekhov AN, Aliev G. Front
Pharmacol. 2020 Apr 8;11:403. doi: 10.3389/fphar.2020.00403. eCollection
2020. PMID: 32322201
3. Signaling Pathways Potentially Responsible for Foam Cell Formation:
Cholesterol Accumulation or Inflammatory Response-What is First? Orekhov
AN, Sukhorukov VN, Nikiforov NG, Kubekina MV, Sobenin IA, Foxx KK,
Pintus S, Stegmaier P, Stelmashenko D, Kel A, Poznyak AV, Wu WK,
Kasianov AS, Makeev VY, Manabe I, Oishi Y.Int J Mol Sci. 2020 Apr
14;21(8):2716. doi: 10.3390/ijms21082716. PMID: 32295185
4. Orekhov AN, Nikiforov NG, Sukhorukov VN, Kubekina MV, Sobenin IA, Wu
WK, Foxx KK, Pintus S, Stegmaier P, Stelmashenko D, Kel A, Gratchev AN,
Melnichenko AA, Wetzker R, Summerhill VI, Manabe I, Oishi Y. Role of
Phagocytosis in the Pro-Inflammatory Response in LDL-Induced Foam Cell
Formation; a Transcriptome Analysis. Int J Mol Sci. 2020 Jan 27;21(3). pii:
E817. doi: 10.3390/ijms21030817. IF=4.183 Q1
5. Galstyan KO, Nedosugova LV, Martirosian NS, Nikiforov NG, Elizova NV,
Kolmychkova KI, Sobenin IA, Orekhov AN. Modification of Tumor Necrosis
Factor-α and C-C Motif Chemokine Ligand 18 Secretion by Monocytes
Derived from Patients with Diabetic Foot Syndrome. Biology (Basel). 2019
Dec 22;9(1). pii: E3. doi: 10.3390/biology9010003. Q1
6. Nikiforov NG, Wetzker R, Kubekina MV, Petukhova AV, Kirichenko TV,
Orekhov AN. Trained Circulating Monocytes in Atherosclerosis: Ex Vivo
Model Approach. Front Pharmacol. 2019 Jun 27;10:725. doi:
10.3389/fphar.2019.00725. eCollection 2019. IF=3.831 Q1
7. Nikiforov NG, Elizova NV, Bukrinsky M, Dubrovsky L, Makeev VJ,
Wakabayashi Y, Liu P, Foxx KK, Kruth HS, Jin X, Zakiev ER, Orekhov AN.
Use of Primary Macrophages for Searching Novel Immunocorrectors. Curr
Pharm Des. 2017 Jan 25. [Epub ahead of print] PubMed PMID: 28124601.
IF=2.832 Q2
8. Nikiforov NG, Zakiev ER, Elizova NV, Sukhorukov VN, Orekhov AN. Multiple-
modified Low-Density Lipoprotein as Atherogenic Factor of patients' Blood:
Development of Therapeutic Approaches to reduce blood Atherogenicity. Curr
Pharm Des. 2017 Jan 24. [Epub ahead of print] PubMed PMID: 28120723.
IF=2.832 Q2
Nikiforov Nikita
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9. Zakiev ER, Nikiforov NG, Orekhov AN. Cell-Based Models for Development
of Antiatherosclerotic Therapies. Biomed Res Int. 2017;2017:5198723. doi:
10.1155/2017/5198723. Review. PubMed PMID: 28286766; PubMed Central
PMCID: PMC5329658. IF= 2.197 Q2
10. Orekhov AN, Pushkarsky T, Oishi Y, Nikiforov NG, Zhelankin AV, Dubrovsky
L, Makeev VJ, Foxx K, Jin X, Kruth HS, Sobenin IA, Sukhorukov VN, Zakiev
ER, Kontush A, Le Goff W, Bukrinsky M. HDL activates expression of genes
stimulating cholesterol efflux in human monocyte-derived macrophages. Exp
Mol Pathol. 2018 Oct;105(2):202-207. doi: 10.1016/j.yexmp.2018.08.003.
Epub 2018 Aug 16. IF= 2.566 Q2
11. Orekhov AN, Oishi Y, Nikiforov NG, Zhelankin AV, Dubrovsky L, Sobenin IA,
Kel A, Stelmashenko D, Makeev VJ, Foxx K, Jin X, Kruth HS, Bukrinsky M1.
Modified Ldl Particles Activate Inflammatory Pathways In Monocyte-Derived
Macrophages: Transcriptome Analysis. Curr Pharm Des. 2018 Sep 11. doi:
10.2174/1381612824666180911120039. IF=2.832 Q2
12. Orekhov AN, Nikiforov NG, Elizova NV, Korobov GA, Aladinskaya AV,
Sobenin IA, Bobryshev YV. Tumor necrosis factor-α and C-C motif chemokine
ligand 18 associate with atherosclerotic lipid accumulation in situ and in vitro.
Curr Pharm Des. 2018 Sep 11. doi: 10.2174/1381612824666180911120726.
IF=2.832 Q2
13. Chistiakov DA, Bobryshev YV, Nikiforov NG, Elizova NV, Sobenin IA,
Orekhov AN. Macrophage phenotypic plasticity in atherosclerosis: The
associated features and the peculiarities of the expression of inflammatory
genes. Int J Cardiol. 2015 Mar 5;184C:436-445. doi:
10.1016/j.ijcard.2015.03.055. [Epub ahead of print] Review. PubMed PMID:
25755062. IF= 3.471 Q2
14. Bobryshev YV, Ivanova EA, Chistiakov DA, Nikiforov NG, Orekhov AN.
Macrophages and Their Role in Atherosclerosis: Pathophysiology and
Transcriptome Analysis. Biomed Res Int. 2016;2016:9582430. doi:
10.1155/2016/9582430. Epub 2016 Jul 17. Review. PubMed PMID:
27493969. IF= 2.197 Q2
15. Orekhov AN, Sobenin IA, Gavrilin MA, Gratchev A, Kotyashova SY, Nikiforov
NG, Kzhyshkowska J. Macrophages in immunopathology of atherosclerosis:
a target for diagnostics and therapy. Curr Pharm Des. 2015;21(9):1172-9.
PubMed PMID: 25312739. IF=2.832 Q2
16. Orekhov AN, Nikiforov NG, Elizova NV, Ivanova EA, Makeev VJ.
Phenomenon of individual difference in human monocyte activation. Exp Mol
Pathol. 2015 Jun 21;99(1):151-154. doi: 10.1016/j.yexmp.2015.06.011. [Epub
ahead of print] PubMed PMID: 26107006. IF= 2.350 Q2
17. Bobryshev YV, Nikiforov NG, Elizova NV, Orekhov AN. Macrophages and
Their Contribution to the Development of Atherosclerosis. Results Probl Cell
Differ. 2017;62:273-298. doi: 10.1007/978-3-319-54090-0_11. Review. IF=
2.12 Q3
18. Nikiforov NG, Kornienko VU, Karagodin VP, Orekhov AN. [Activation of
macrophages in atherosclerosis. Message 1: Activation of macrophages in
normal and in atherosclerotic lesions]. Pathological physiology and
experimental therapy 2015 (3) 128-131 PMID: 29215250, Russian, IF = 0.16
Q4
Chapter in the book “Advances in Lipoprotein Research”
Nikiforov NG, Zakiev ER, Melnichenko AA, Orekhov AN. Circulating
Atherogenic Multiple-Modified Low-Density Lipoprotein: Pathophysiology and
Clinical Applications, Advances in Lipoprotein Research, Prof. Turgay Isbir
(Ed.), InTech, 2017 DOI: 10.5772/67055.
Nikiforov Nikita
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Experience in managing and participating in research projects
Head:
Russian Foundation for Basic Research, 18-34-00997, Study of the role of genes
identified by transcriptome analysis of cholesterol-loaded macrophages in key
molecular mechanisms of progression of atrosclerosis, 2018-2019
Participant:
Grants Council of the President of the Russian Federation, NSh-6995.2016.7, Use of
transgenic cells to study the mechanisms of communication between atherosclerosis
and defects of the mitochondrial genome, 2016-2017
Fund for the Promotion of the Development of Small Forms of Enterprises in the
Scientific and Technical Field, 12582р / 24022, Development and creation of a
diagnostic kit for measuring the level of modified low density lipoproteins in human
blood, 2014-2016
Russian Science Foundation, 14-14-01038, Variability of the mitochondrial genome:
effect on the functional activity of cells, 2014-2016
Russian Science Foundation, 14-15-00245, Molecular Mechanism and
Pathophysiological Importance of Modifying Proteins with Active Carbonyl
Compounds, 2014-2016 *
Russian Science Foundation, 14-15-00112, Transcriptome Activated Macrophage
Study, 2014-2016
Russian Science Foundation, 14-15-00112, Transcriptome Activated Macrophage
Study, 2017-2018 (extension)
Russian Foundation for Basic Research, 14-04-01920, Effect of hyperglycemia on
STAT-dependent signaling in macrophages of types 1 and 2 and plasticity of the
macrophage phenotype, 2014-2016
* Excluded from project No. 14-15-00245 in 2016
Key scientific results over the past 5 years
It has been demonstrated that modified LDL circulating in the blood of patients with
atherosclerosis, in turn, is able to modulate the immune response.
Genes involved in the innate immunity reaction have been identified that are
activated due to the interaction of monocytes / macrophages with modified LDL and
subsequently induce the accumulation of cholesterol.
There are preliminary unpublished data suggesting that circulating monocytes of
patients with atherosclerosis are more susceptible to pro-inflammatory stimuli, i.e.
more activated. Such enhanced activation of monocytes in patients with
atherosclerosis may be the result of training of monocytes.
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In a pilot study, a relationship was established between the blood lipid profile and the
ability of circulating monocytes to activate.
Hobbies
Piano, pictorial art, soccer, basketball.